Mitosis: Springtime for Chromatin

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Mitosis: Springtime for Chromatin

When a eukaryotic cell divides, tension builds at centromeres as spindle forces pull chromosomes toward opposite poles during metaphase. New data show that centromeric chromatin stretches in response to these forces, revealing a mechanical role for chromatin packaging in mitosis.

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Pericentric Chromatin Is Organized into an Intramolecular Loop in Mitosis

BACKGROUND Cohesin proteins link sister chromatids and provide the basis for tension between bioriented sister chomatids in mitosis. Cohesin is concentrated at the centromere region of the chromosome despite the fact that sister centromeres can be separated by 800 nm in vivo. The function of cohesin at sites of separated DNA is unknown. RESULTS We provide evidence that the kinetochore promote...

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Metaphase chromosomes are visible hallmarks of mitosis, yet our understanding of their structure and of the forces shaping them is rudimentary. Phosphorylation of histone H3 serine 10 (H3 S10) by Aurora B kinase is a signature event of mitosis, but its function in chromatin condensation is unclear. Using genetically encoded ultraviolet light-inducible cross-linkers, we monitored protein-protein...

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ژورنال

عنوان ژورنال: Current Biology

سال: 2007

ISSN: 0960-9822

DOI: 10.1016/j.cub.2007.04.019